def insert_sample_data(self, values): # added self since you are referencing it below
 with self.con.cursor() as cur:
  sql = "insert into sampletable values (%s, %s, %s)" # Use %s for parameters
  cur.executemany(sql, values) # Pass the list of tuples directly
  self.con.commit()
 
list1 = [(1100, 'abc', '{"1209": "Y", "1210": "Y"}'), (1100, 'abc', None)]
self.insert_sample_data(list1) # pass the list directly

import pymysql 
class Connection: 
 def __init__(self):
  self.host = 'localhost'
  self.user = 'nameit'
  self.password = 'YES'
  self.port = 3306
  self.db = 'Xomai' 
 
 def connection(self): 
  db = pymysql.connect(host=self.host, user=self.user, password=self.password, port=self.port, db=self.db)
  cur = db.cursor()
  return db, cur
 
 def create_table(self, cur): 
  sql = """CREATE TABLE `activity_feedback` (
     `id` bigint(20) NOT NULL AUTO_INCREMENT,
     `inst_id` bigint(20) DEFAULT NULL COMMENT 'ID',
     `broadcast_id` bigint(20) DEFAULT NULL COMMENT '你好',
     `student_id` bigint(20) DEFAULT NULL COMMENT '學生ID',
     `content` varchar(1024) DEFAULT NULL COMMENT '學員內容',
     `comment` varchar(255) DEFAULT NULL COMMENT '注釋',
     `gmt_create` datetime DEFAULT NULL,
     `gmt_modify` datetime DEFAULT NULL,
     PRIMARY KEY (`id`),
     KEY `activity_feedback_student_id_index` (`student_id`)
    ) ENGINE = InnoDB AUTO_INCREMENT = 1050 DEFAULT CHARSET = utf8mb4 COMMENT = '學員表'"""
  cur.execute(sql)
 def insert(self, id, inst_id, broadcast_id, student_id, content, comment, gmt_create, gmt_modify):
  sql = """INSERT INTO `activity_feedback` (
     `id`, `inst_id`, `broadcast_id`, `student_id`, `content`, `comment`, `gmt_create`, `gmt_modify`)
     VALUES ('{}','{}','{}','{}','{}','{}','{}','{}')""".format(id, inst_id, broadcast_id, student_id, content, comment, gmt_create, gmt_modify)
  try:
   self.connection()[1].execute(sql)
   self.connection()[0].commit()
  except:
   self.connection()[0].rollback()
if __name__ == '__main__':
 conn = Connection()
 conn.insert(123, 123, 324, 3451, 'ajdf', 'sdfs', '2013-2-5', '2014-3-4')

  try:
   self.connection()[1].execute(sql)
   self.connection()[0].commit()
  except:
   self.connection()[0].rollback()

  try:
   cons = self.connection()
   cons[1].execute(sql)
   cons[0].commit()
   cons[0].close()
  except:
   cons[0].rollback()